Search Database

Select Protein (1 found)

UniProt ID Gene Symbol Protein Name Organism Length Action
B4DNK4 PKM Pyruvate kinase (EC 2.7.1.40) Homo sapiens (Human) 457 aa

Protein Details: B4DNK4 (PKM)

Protein Information
AccessionB4DNK4
Protein NamesPyruvate kinase (EC 2.7.1.40)
Gene SymbolPKM
OrganismHomo sapiens (Human)
Length457 aa
IsoformsNo isoforms
Related PMIDs 31251020 32944167 36430497 31251020 (mass) 32651440 (mass) 36430497 (mass)
Database SourcesCysModDBdbPTMSwissPalm
These studies detected palmitoylation of this protein in the samples.
Protein Sequence
Types:
Experimental Database High Prediction Non-palmitylated Cys
1-501MSKPHSEAGT11AFIQTQQLHA21AMADTFLEHM31CRLDIDSPPI41TARNTGIICT
51-10051IGPASRSVEL61KKGATLKITL71DNAYMEKCDE81NILWLDYKNI91CKVVEVGSKI
101-150101YVDDGLISLQ111VKQKGADFLV121TEVENGGSLG131SKKGVNLPGA141AVDLPAVSEK
151-200151DIQDLKFGVE161QDVDMVFASF171IRKASDVHEV181RKVLGEKGKN191IKIISKIENH
201-250201EGVRRFDEIL211EASDGIMVAR221GDLGIEIPAE231KVFLAQKMMI241GRCNRAGKPV
251-300251ICATQMLESM261IKKPRPTRAE271GSDVANAVLD281GADCIMLSGE291TAKGDYPLEA
301-350301VRMQHLIARE311AEAAIYHLQL321FEELRRLAPI331TSDPTEATAV341GAVEASFKCC
351-400351SGAIIVLTKS361GRSAHQVARY371RPRAPIIAVT381RNPQTARQAH391LYRGIFPVLC
401-450401KDPVQEAWAE411DVDLRVNFAM421NVGKARGFFK431KGDVVIVLTG441WRPGSGFTNT
451-457451MRVVPVP
Palmitoylation Sites Details
Position Database Domains Literature (PMID/Cell-Tissue) Mass(PMID/Cell-Tissue) Prediction Scores
31 Pyruvate kinase, C-terminal domain superfamily -
Unknown (32651440)
cerebral cortex (36430497)
Deep-Palm: 0.40
49 -
HeLa (29575903)
LNCaP (31251020)
Unknown (32651440)
cerebral cortex (36430497)
GPS-Palm: 0.89
Deep-Palm: 0.91
78 - -
LNCaP (31251020)
Unknown (32651440)
cerebral cortex (36430497)
Deep-Palm: 0.34
91 - - -
GPS-Palm: 0.72
Deep-Palm: 0.67
243 - - -
GPS-Palm: 0.91
Deep-Palm: 0.89
252 - -
Unknown (32651440)
cerebral cortex (36430497)
GPS-Palm: 0.86
Deep-Palm: 0.77
284 Pyruvate kinase, barrel domain
HeLa (29575903)
Unknown (32651440)
cerebral cortex (36430497)
GPS-Palm: 0.81
Deep-Palm: 0.85
349 Pyruvate kinase, alpha/beta domain
HeLa (29575903)
cerebral cortex (36430497)
GPS-Palm: 0.92
Deep-Palm: 0.96
350 Pyruvate kinase, alpha/beta domain
HeLa (29575903)
cerebral cortex (36430497)
GPS-Palm: 0.95
Deep-Palm: 0.95
400 SWISSPALM DBPTM CYSMODDB Pyruvate kinase alpha/beta domain
HeLa (29575903)
Unknown (32651440)
cerebral cortex (36430497)
Deep-Palm: 0.94
Score Interpretation:
GPS-Palm: Thresholds - High (≥0.8920), Medium (≥0.7766), Low (≥0.6484), Very Low (<0.6484)
Deep-Palm: Higher score indicates higher probability of palmitoylation (High ≥0.9)
Tissue/Cell Line Expression
Literature Data - Tissue/Cell Line Expression
Tissue Specificity Index (TSI): 0.364
4
PC3
Specificity: 0.250
4/4 (100.0%)
4
cerebral cortex
Specificity: 0.250
4/4 (100.0%)
3
LNCaP
Specificity: 0.188
3/3 (100.0%)
Mass Spectrometry Data - Tissue/Cell Line Expression
Tissue Specificity Index (TSI): 0.800
4
Cerebral Cortex (Mass)
Specificity: 0.250
4/4 (100.0%)
1
LNCaP cells (Mass)
Specificity: 0.062
1/4 (25.0%)
Palmitoylation Distribution by Study and Tissue/Cell Line
Chart Explanation: Each bar represents a study (PMID). Blue bars: Literature data, Orange bars: Mass Spectrometry data. The colored bottom segment shows palmitoylated samples, while the gray top segment shows non-palmitoylated samples.
Conservation score for cysteine
PhyloP for Cysteine
PhastCons Conservation Scores for Cysteine
TCGA Cysteine Mutation Information

Note: Mutations indicate amino acid changes that may create potential palmitoylation sites.

Position Amino Acid Change Frequency Type Function Cancer Type
32 R → C 0.002288 SNP Missense Mutation STAD
49 C → Y 0.002288 SNP Missense Mutation STAD
92 R → C 0.001887 SNP Missense Mutation UCEC
158 W → C 0.010870 SNP Missense Mutation ACC
298 G → C 0.001887 SNP Missense Mutation UCEC
307 F → C 0.007299 SNP Missense Mutation READ
317 C → Y 0.001014 SNP Missense Mutation BRCA
376 R → C 0.002545 SNP Missense Mutation GBM
467 R → C 0.002288 SNP Missense Mutation STAD
467 R → C 0.002033 SNP Missense Mutation LUSC
516 R → C 0.001887 SNP Missense Mutation UCEC